New MRSA real-time PCR kit
New MRSA real-time PCR kit

medac Diagnostics offers a new real-time PCR kit, which detects the methicillin resistant Staphylococcus aureus (MRSA).
The MRSA detection is based on the amplification of three specific loci (the nuc gene, the SCCmec/orfX junction region and the resistance genes mecA/mecC) and the measurement of the fluorescence increase. The presence of the nuc gene is indicated by the TEX fluorophore fluorescence growth, of the SCCmec/orfX junction by the FAM fluorophore and of the mecA/mecC genes by the Cy5 fluorophore fluorescence growth.
An Internal Standard (IS) is either included in the ready to use Mastermix, controlling the possible inhibition of the PCR (ISIN version) or needs to be added to the sample, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The “hot start” technology minimizes non-specific reactions, assuring maximum sensitivity and Uracil-DANN-glycosylase (UDG) eliminates possible contaminations of the PCR by amplification products.
Validated specimen are aspirate, sputum, swab and urine. The kit is designed for in vitro diagnostics and provides qualitative detection.

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