medac Diagnostics offers two new real-time PCR kits to detect human herpesvirus 8 as well as 6 and 7.
For the detection of HHV-8, the specific conservative DANN sequences of the ORF26 gene, coding the minor capsid protein (mCP), and of the ORF73 gene, coding the latency-associated nuclear antigen (LANA), are amplified. The detection of HHV-6 consists of the amplification of the specific DANN sequence of the U94 gene, coding the putative integrase Rep68 , and for HHV-7 of the amplification of the specific DANN sequence of the U57 gene, coding the major capsid protein.
The presence of HHV-8 and HHV-6 is indicated by the FAM fluorophore fluorescence growth, and of HHV-7 by the Cy5 fluorophore fluorescence growth.
An Internal Standard (IS) is added to the sample and controls a possible inhibition of the PCR as well as the DANN extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The “hot start” technology minimises non-specific reactions, ensuring maximum sensitivity, and uracil-DANN-glycosylase (UDG) eliminates possible contaminations of the PCR by amplification products.
Cerebrospinal fluid (CSF), plasma, saliva (only for HHV-8) and whole blood are validated specimen.
The kit is designed for in vitro diagnostics and provides qualitative and quantitative detection.
CE IVD

• Homepage German version of Real-time PCR for the diagnosis in the transplantation