One duplex kit for the detection of both BKV and JCV and one monoplex kit for the detection of JCV.
   
The detection is based on the amplification of the specific conservative DNA sequence between the genes for VP1 and VP2 protein and measuring the concentration increase of the amplification product during the PCR process by means of a fluorescence-labeled probes. In the duplex kit BKV presence is indicated by FAM fluorophore and the JCV presence is indicated by Cy5 fluorophore fluorescence increase. An Internal Standard (IS) is included in the reaction mix, controlling the possible inhibition of the PCR reaction (ISIN version) or also the quality of DNA extraction (ISEX version). IS positive amplification is detected in the HEX fluorescence channel.
The detection kits take an advantage of the “hot start” technology, minimizing non-specific reactions and assuring maximum sensitivity. It contains Uracil-DNA-Glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. Validated for the use of EDTA whole blood, urine, and cerebrospinal fluid. The kit is designed for In-vitro diagnostics and provides qualitative and quantitative detection. IVD CE