medac Diagnostics offers a new Adenovirus quantitative real-time PCR kit.
The detection is based on the amplification of the highly conservative DNA sequence (E2B gene) and on measuring the concentration increase in the amplification product during the PCR process by means of a fluorescence-marked probes. An Internal Standard (IS) is included in the reaction mix, controlling the possible inhibition of the PCR reaction (ISIN version) or also the quality of DNA extraction (ISEX version). IS positive amplification is detected in the HEX fluorescence channel.
The detection kit takes advantage of the “hot start” technology, minimizing non-specific reactions and assuring maximum sensitivity. It contains Uracil-DNA-Glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. EDTA whole blood, plasma, urine, stool, and nasopharyngeal swabs may be used for Adenovirus detection with this kit. It is designed for In-vitro diagnostics and provides qualitative and quantitative detection.
IVD CE