medac Diagnostics offers a new PAI-1 real-time PCR kit.
The method is based on amplification and detection of target sequence using allele specific fluorophore labelled probes. The target sequence is the insertion-deletion polymorphism 4G/5G. The presence of wild-type allele (5G/5G) is detected in FAM fluorescent channel and mutant allele (4G/4G) in HEX fluorescent channel. In case of heterozygous genotype (4G/5G) signal is detected in both channels. The detection kit contains a ready to use Master Mix and utilizes hot start PCR, minimizing non-specific reactions and assuring maximum sensitivity. The kit is designed for in vitro diagnostics.
IVD CE