medac Diagnostics offers a new Vancomycin-Resistant-Enterococci real-time PCR kit.
The detection is based on the amplification of a specific conservative DNA sequence in the area of the vanA and vanB genes of Enterokokkus faecalis and Enterococcus faecium using fluorophore labelled probes. VanA gene presence is indicated by FAM fluorophore fluorescence growth and the presence of vanB gene is indicated by Cy5 fluorophore fluorescence growth. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of the PCR (ISIN version) or excluded, controlling also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit utilizes the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR by amplification products. The kit performs very sensitive VRE detection in clinical material. The kit is designed for in vitro diagnostics and provides qualitative detection.
CE IVD