New Aspergillus real-time PCR kit

medac Diagnostics offers a new real-time PCR kit, which detects the clinically significant Aspergillus species (A. fumigatus, A. flavus, A. niger and A. terreus).
The detection is based on the amplification of a specific sequence of ribosomal DNA (rDNA) using fluorophore labelled probes. Aspergillus spp. presence is indicated by FAM fluorophore fluorescence growth and the presence of Aspergillus terreus is indicated by Cy5 fluorophore fluorescence growth. An Internal Standard (IS) is included in the reaction mix, controlling the possible inhibition of the PCR reaction (ISIN version) and possibly also the DNA extraction process quality (ISEX version). IS positive amplification is detected in the HEX fluorophore fluorescence channel. The detection kit utilizes the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products.
The kit is validated for whole blood, plasma, serum, CSF, Sputum and BAL.
The PCR assay is designed for in vitro diagnostics and provides qualitative detection.

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