New Ureaplasma (UUP) real-time PCR kit

medac Diagnostics offers a new real-time PCR kit detecting U. urealyticum and U. parvum.
The detection is based on the amplification of the conservative sequence of the UreD gene using fluorophore labelled probes. The presence of Ureaplasma parvum is indicated by the FAM fluorophore fluorescence growth. Ureaplasma urealyticum detection is indicated by the fluorescence growth in the FAM and Cy5 channels. An Internal Standard (IS) is either included in the reaction mix, controlling the possible inhibition of PCR (ISIN version) or the DNA isolation quality and ihibition of PCR (ISEX version). IS positive amplification is detected in the fluorescence channel for the HEX fluorophore. The detection kit utilizes the "hot start" technology, minimizing non-specific reactions and assuring maximum sensitivity. Ready to Use MasterMix contains uracil-DNA-glycosylase (UDG), eliminating possible contamination of the PCR reaction by amplification products. The kit is validated for urine specimen and swabs. The real-time PCR kit is designed for in vitro diagnostics and provides qualitative detection.

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